Int J Biochem Mol Biol 2011;2(3):274-286

Original Article
Effect of different carbon sources on cellulase production by Hypocrea jecorina
(Trichoderma reesei) strains

Mehdi Dashtban, Robert Buchkowski, Wensheng Qin

Biorefining Research Initiative, Lakehead University, 955 Oliver Road, Thunder Bay, ON P7B 5E1 Canada; Department of Biology, Lakehead
University, 955 Oliver Road, Thunder Bay, ON P7B 5E1 Canada.

Received August 9, 2011; accepted August 26, 2011; Epub September 9, 2011; Published August 30, 2011

Abstract: The ascomycete Hypocrea jecorina, an industrial (hemi)cellulase producer, can efficiently degrade plant polysaccharides. At present,
the biology underlying cellulase hyperproduction of T. reesei, and the conditions for the enzyme induction, are not completely understood. In the
current study, three different strains of T. reesei, including QM6a (wild-type), and mutants QM9414 and RUT-C30, were grown on 7 soluble and
7 insoluble carbon sources, with the later group including 4 pure polysaccharides and 3 lignocelluloses. Time course experiments showed
that maximum cellulase activity of QM6a and QM9414 strains, for the majority of tested carbon sources, occurred at 120 hrs, while RUT-C30
had the greatest cellulase activity around 72 hrs. Maximum cellulase production was observed to be 0.035, 0.42 and 0.33 µmol glucose
equivalents using microcrystalline celluloses for QM6a, QM9414, and RUTC-30, respectively. Increased cellulase production was positively
correlated in QM9414 and negatively correlated in RUT-C30 with ability to grow on microcrystalline cellulose. (IJBMB1107002)

Keywords: Trichoderma reesei, cellulase, growth characterization, carbon sources

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Address all correspondence to:
Dr. Wensheng Qin
Biorefining Research Initiative
Lakehead University, 955 Oliver Rd
Thunder Bay, Ontario P7B5E1, Canada                                                                                                        
Tel:  (807) 343-8840, Fax:  (807) 346-7796