Int J Biochem Mol Biol 2011;2(4):309-317

Original Article
Physicochemical properties and acute toxicity studies of a lectin from the saline extract
of the fruiting bodies of the shiitake mushroom, Lentinula edodes (Berk)

Queensley Eghianruwa, Oludele Odekanyin, Adenike Kuku

Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria

Received September 28, 2011; accepted October 24, 2011; Epub November 10, 2011; Published December 15, 2011

Abstract: A lectin (LEL) was isolated from the fresh fruiting bodies of the shiitake mushroom Lentinula edodes by a combination of gel filtration
chromatography on Sephadex G-150 and affinity chromatography on an N-acetyl-D-galactosamine-Sepharose 4B column. Its molecular mass,
as determined by gel filtration, was estimated to be 71, 000 Daltons and its structure is homotetrameric with subunit molecular weight of
approximately 18,000 Daltons. LEL agglutinated non-specifically red blood cells from the human ABO system as well as rabbit erythrocytes
and in haemagglutination inhibition assays, exhibited sugar-binding specificity toward N-acetyl-D-galactosamine. EDTA had no inhibitory effect
on its haemagglutinating activity, which was stable up to 70 °C and was not affected by changes in pH. The lectin had no covalently-linked
carbohydrate and amino acid composition analysis revealed that it contained 124 amino acid residues and was rich in tyrosine, proline,
phenylalanine, arginine, glutamic acid and cysteine. LEL did not cause mortality neither was it observed to alter the morphology of key organs
when administered intraperitoneally at concentrations up to 10,000 mg kg-1 body weight of mice. (IJBMB1109002)

Keywords: Lectin, Lentinula edodes, N-acetyl-D-galactosamine, shiitake mushroom, fruiting bodies, agglutination

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Address all correspondence to:
Dr. Queensley Eghianruwa (E-mail: adenikekuku@gmail.com)
Dr. Oludele Odekanyin (E-mail: akuku@oauife.edu.ng)
Dr. Adenike Kuku (adenikekuku@yahoo.com)
Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.
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