Int J Biochem Mol Biol 2012;3(3):282-289
Review Article
Hydroxyl radical generation theory: a possible explanation of unexplained actions of
mammalian catalase
Madhur M Goyal, Anjan Basak
Department of Biochemistry, J.N. Medical College, Datta Meghe Institute of Medical Sciences (Deemed University), Wardha-442004, India
Received July 24, 2012; Accepted September 17, 2012; Epub September 25, 2012; Published September 30, 2012
Abstract: Catalase is well known antioxidant enzyme which catalyses the dissociation of hydrogen peroxide directly into H2O and O2.
Mammalian catalase has been considered as ‘a venerable enzyme with new mysteries’. Some aspects of its mechanism of action are
mystifying and many of new findings are still unexplained. To fill up the gap we propose the ‘Hydroxyl Radical Generation Theory (HRGT)’ with
possible mechanism. According to HRGT, mammalian catalase apart from its known catalytic reaction generates hydroxyl radicals (HRs). The
HR generation mainly depends on concentration of specific substrate, hydrogen peroxide. The present theory is supported by previous
experimental findings and has great deal of observational evidences. The proposed mechanism of generation of HRs answer several
unexplained features of mammalian catalase, however, should be tested further. (IJBMB1207003).
Keywords: Mammalian catalase, mechanism of action, Hydroxyl Radical Generation Theory (HRGT), role of NADPH
Address all correspondence to:
Dr. Madhur M Goyal
Department of Biochemistry
J. N. Medical College,
Datta Meghe Institute of Medical Sciences (Deemed University),
Wardha-442004, India.
Telephone: +91 9823585404
E-mail: monusvm@yahoo.com
Review Article
Hydroxyl radical generation theory: a possible explanation of unexplained actions of
mammalian catalase
Madhur M Goyal, Anjan Basak
Department of Biochemistry, J.N. Medical College, Datta Meghe Institute of Medical Sciences (Deemed University), Wardha-442004, India
Received July 24, 2012; Accepted September 17, 2012; Epub September 25, 2012; Published September 30, 2012
Abstract: Catalase is well known antioxidant enzyme which catalyses the dissociation of hydrogen peroxide directly into H2O and O2.
Mammalian catalase has been considered as ‘a venerable enzyme with new mysteries’. Some aspects of its mechanism of action are
mystifying and many of new findings are still unexplained. To fill up the gap we propose the ‘Hydroxyl Radical Generation Theory (HRGT)’ with
possible mechanism. According to HRGT, mammalian catalase apart from its known catalytic reaction generates hydroxyl radicals (HRs). The
HR generation mainly depends on concentration of specific substrate, hydrogen peroxide. The present theory is supported by previous
experimental findings and has great deal of observational evidences. The proposed mechanism of generation of HRs answer several
unexplained features of mammalian catalase, however, should be tested further. (IJBMB1207003).
Keywords: Mammalian catalase, mechanism of action, Hydroxyl Radical Generation Theory (HRGT), role of NADPH
Address all correspondence to:
Dr. Madhur M Goyal
Department of Biochemistry
J. N. Medical College,
Datta Meghe Institute of Medical Sciences (Deemed University),
Wardha-442004, India.
Telephone: +91 9823585404
E-mail: monusvm@yahoo.com
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