Int J Biochem Mol Biol 2013;4(1):67-74
Identification and association of TGFβ-1 expression in patients with asthma in a Polish
population - Lodz metropolitan area study
Michał Panek, Tadeusz Pietras, Janusz Szemraj, Artur Fabijan, Piotr Kuna
Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz, 22 Kopcinskiego Str., 90-153 Lodz, Poland; Department of
Pneumology and Allergology, Medical University of Lodz, 22 Kopcinskiego Str., 90-153 Lodz, Poland; Department of Medical Biochemistry,
Medical University of Lodz, 6/8 Mazowiecka Str., Lodz 92-215, Poland
Received March 17, 2013; Accepted March 25, 2013; Epub March 31, 2013; Published April 15, 2013
Abstract: Objective: Interaction of genotype and environment results in a specific phenotype of the clinical course of asthma. TGFβ-1 gene
belongs to the important group of genes involved in the regulation of proliferation, differentiation, adhesion, and migration of a variety of cell
types. TGFβ-1 is inhibitory for B and T cells, as well as IgE production. In particular, it is engaged in inflammation of the bronchi and airway
remodeling in asthma, which processes are critical in the pathogenesis of the disease. The aim of this study was to evaluate the correlation
between the level of expression of TGFβ-1 and the severity of asthma. Methods: The study included 39 participants (20 healthy subjects and 19
patients with asthma). Each sample was analysed by using real time PCR. Results: There was statistical associations between the control
group and the group of patients (p = 0,00007). It was demonstrated strong correlation between healthy and patients with severe asthma
according GINA guidelines (p = 0,017). It was found the strong statistical correlation between healthy and patients with severe corticosteroid
dependent asthma (p = 0,013). Correlations were observed between levels of asthma severity according to the ATS guidelines and controls.
The influence of the level of TGFβ-1 mRNA expression and the severity of asthma (ATS) in the FEV1 (%) parameter value was found.
Conclusion: It was found that an important role is played by TGFβ-1 in the pathogenesis of asthma. (IJBMB1303003).
Keywords: Asthma, inflammation, airway obstruction, transforming growth factor β-1, T regulatory cells
Address correspondence to: Dr. Michał Panek, Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz, 90-153 Lodz,
Poland, Kopcińskiego 22 Str. Phone: 00 48 42 677 69 49 or 00 48 42 677 69 39; E-mail: firstname.lastname@example.org